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Image Search Results
Journal: bioRxiv
Article Title: Evasion of serum antibodies and complement by Salmonella Typhi and Paratyphi A
doi: 10.1101/2025.01.20.633845
Figure Lengend Snippet: ( A) Restoration of S . Paratyphi A long and O9-antigen increases human C3a and (B) C5a production following incubation with human serum for 60 min at 37°C. The S. Typhi Vi capsule prevents C3a production but does not impact C5a release. Long O-antigen in S. Typhimurium is the primary determinant of C5a release. C3a and C5a release were measured by enzyme-linked immunosorbent assay (ELISA). For statistical comparison, individual replicates are normalized to the corresponding wild-type serovar and statistical analysis performed by one sample t test with a hypothetical value of 1.0, with P values in red indicating statistical significance as P < 0.05. Column bars represent the means, with error bars showing standard deviation. STm, S. Typhimurium; STy, S. Typhi; SPa, S. Paratyphi A.
Article Snippet: Human C3a and C5a production were measured using the
Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Comparison, Standard Deviation
Journal: bioRxiv
Article Title: Evasion of serum antibodies and complement by Salmonella Typhi and Paratyphi A
doi: 10.1101/2025.01.20.633845
Figure Lengend Snippet: ( A) O-antigen modifications by glycosyltransferases in S. Paratyphi A decrease complement activation as measured by C5a release and (B) IgM binding. (C) and (D) O-antigen modifications by glycosyltransferases in S. Paratyphi A do not impact complement deposition or (E) serum resistance. (F) O-antigen modifications by glycosyltransferases in S. Typhimurium do not impact complement activation, (H) and (I) C3b deposition, or (J) serum resistance, but (G) reduce IgM binding. Statistical analysis was performed by one-way ANOVA on 3-4 independent replicates, with P values in red indicating statistical significance with P <0.05. Column bars represent the means, with error bars displaying standard deviation. STm, S. Typhimurium; STy, S. Typhi; SPa, S. Paratyphi A.
Article Snippet: Human C3a and C5a production were measured using the
Techniques: Activation Assay, Binding Assay, Standard Deviation
Journal: PLoS ONE
Article Title: In vitro hemo- and cytocompatibility of bacterial nanocelluose small diameter vascular grafts: Impact of fabrication and surface characteristics.
doi: 10.1371/journal.pone.0235168
Figure Lengend Snippet: (A) Plasma levels of C3a. (B) Plasma levels of TCC. The values represented are relative to the baseline C3a and TCC levels immediately upon blood drawing. N = 6 (PET, ePTFE, INV, PAD, SAC), n = 7 (OIS, STD), n = 44 (CTRL).
Article Snippet: Circulating plasma levels of TAT and complement factors such as terminal complement complex (TCC) and anaphylatoxin (C3a) were quantified using commercially available enzyme-linked immunosorbent assay (ELISA) kits according to manufacturers’ instructions (TAT: Catalogue No #108907; Abcam plc, Cambridge, UK;
Techniques: